Calu-3 人类肺腺癌细胞（胸水）
| ATCC® Number: | HTB-55™ |
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| Designations: | Calu-3 |
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| | Depositors: | J Fogh |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Homo sapiens (human) |
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| | Morphology: | epithelial
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| | Source: | Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Calu-3 人类肺腺癌细胞（胸水） |
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| | Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439. |
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| | Applications: | transfection host (Roche FuGENE® Transfection Reagents) |
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| | Tumorigenic: | Yes |
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| | Antigen Expression: | Blood Type A; Rh+ |
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| | DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 12,14 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 16,17 |
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| | Cytogenetic Analysis: | The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations. |
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| | Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
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| | Age: | 25 years |
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| | Gender: | male |
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| | Ethnicity: | Caucasian |
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| | Comments: | The patient had received prior therapy with cytoxan, bleomycin and adriamycin. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
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| | Subculturing: | Protocol:Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
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MLE-12 小鼠肺上皮细胞
saos-2 人成骨肉瘤细胞
MA104 非洲绿猴胚胎肾上皮细胞
ball-1 细胞
nci-h226 人肺鳞癌细胞
MCA38 小鼠结肠癌细胞
LTPA 小鼠胰腺癌细胞
NCI-H23 人非小细胞肺癌细胞
293.00 人胚肾细胞
293FT 人胚肾细胞-用于慢病毒包装
293TN 人胚肾细胞--用于慢病毒包装
293AV 人胚肾细胞-用于腺病毒包装
293T 人胚肾细胞
208F 大鼠成纤维细胞
786-O 人肾透明细胞腺癌细胞
A172 人脑胶质母细胞瘤细胞
A549 人肺癌细胞 星空体育游戏下载官方购买
AGS 人胃癌细胞
BEL7402 人肝癌星空体育游戏下载官方
AZ-521 人胃癌细胞
B16F0 小鼠黑色素瘤细胞
Bel-7402 人肝癌细胞
Bel-7404 人肝癌细胞
BGC-823 人胃癌细胞
C6 大鼠脑胶质瘤细胞
SW480 人结肠腺癌细胞
CaCo-2 人结肠癌细胞
Cho-K1 仓鼠卵巢细胞亚株
COS-7 非洲绿猴SV40转化的肾细胞
GES-1 人胃上皮细胞
HCT-8 人回盲肠癌细胞
HeLa
Hepa1-6 小鼠肝癌细胞
HepG2 人肝癌细胞
hFOB1.19 人永生化成骨细胞
HGC-27 人胃癌细胞
Hs 746T 人胃癌细胞
HT-29 人结肠癌细胞
KB 人口腔表皮样癌细胞
LoVo 人结肠癌细胞
LLC-PK1 猪肾近曲小管上皮细胞
MCF-10A 人正常乳腺上皮细胞
MCF-7 人乳腺癌细胞
MDA-MB-231 人乳腺癌细胞
MGC80-3 人胃癌细胞
MKN-45 人胃癌细胞
NCI-H460 人大细胞肺癌细胞
NIH-3T3 小鼠成纤维细胞
NUGC-3 人胃癌细胞
Raw264-7 小鼠单核细胞白血病细胞
SGC-7901 人胃癌细胞
SMMC-7721 人肝癌细胞
SNU-638 人胃癌细胞
SNU-739 人肝癌细胞
Tca8113 人舌鳞癌细胞 星空体育游戏下载官方购买
U251 人脑胶质瘤细胞
U-373MG 人脑神经胶质瘤细胞
U-87 MG 人脑星形胶质母细胞瘤
BT-474 人乳腺导管癌细胞
PANC-1 人胰腺癌细胞
HEK293 人胚肾细胞
Hep3B 人肝癌细胞
HL-7702 人正常肝细胞
Raw-Blue 小鼠单核细胞白血病细胞
WEHI-231 小鼠B淋巴细胞
OPM-2 人骨髓瘤细胞
NCI-H929 人骨髓瘤细胞
U266 人骨髓瘤细胞
EJ 人膀胱癌细胞
HOC 大鼠肝卵圆细胞
RWPE-1 人正常前列腺上皮细胞
CAL 27 人舌鳞癌细胞
Calu-3 人肺腺癌细胞
Eca-109 人食管癌细胞
DLD-1 人结直肠腺癌上皮细胞
5637.00 人膀胱癌细胞
SK-OV-3 人卵巢癌细胞
GBC-SD 人胆囊癌细胞
BEL7402 人肝癌星空体育游戏下载官方
SW620 人结肠癌细胞
MRC-5 人胚肺成纤维细胞 星空体育游戏下载官方购买
PC9 人肺癌细胞
PC14 人肺癌细胞
BxPC 人原位胰腺腺癌细胞
PK（15） 猪肾上皮细胞
MDCK 狗肾上皮细胞
3D4/21 猪肺泡巨噬细胞
MHCC-97L 人肝癌细胞（低转移）
MHCC-97H 人肝癌星空体育游戏下载官方（高转移）
MiaCaPa-2 人胰腺癌细胞
HN13 口腔鳞状细胞癌细胞
Bend3 小鼠脑微血管内皮细胞
QBC939 人胆管癌细胞
Ishikawa 人子宫内膜癌细胞
A498 人肾癌细胞
143B
WEHI-231 小鼠B淋巴细胞
H322 人非小细胞肺癌细胞
Panc O2
S8 BEL7402 人肝癌星空体育游戏下载官方
R5 星空体育游戏下载官方购买
Pig MSC 猪骨髓间质干细胞
HCAEC 人冠状动脉内皮细胞
marc-145 猴胚胎肾上皮细胞
3D4/21 猪肺泡巨噬细胞
PK（15） 猪肾上皮细胞
MADB-106 大鼠乳腺癌细胞
Sf9 昆虫卵巢细胞
DADY
HUVEC[HUV-EC-C] 人脐静脉内皮细胞
PC14 人肺癌细胞
PC9 人肺癌细胞
Eca-109 人食管癌细胞
CAL 27 人舌鳞癌细胞
HOC 肝卵圆细胞
EJ 人膀胱癌细胞
U266 人骨髓瘤细胞
293-mTLR5 人胚肾细胞
Raw-Blue 小鼠单核细胞白血病细胞
HL-7702 人正常肝细胞
Hep3B 人肝癌细胞
U-373MG 人脑神经胶质瘤细胞
Tca-8113 人舌鳞癌细胞
SNU-739 人肝癌细胞
SNU-638 人胃癌细胞
NUGC-3 人胃癌细胞
NIH-3T3 小鼠成纤维细胞
MKN-45 人胃癌细胞
LLC-PK1 猪肾近曲小管上皮细胞
Hs 746T 人胃癌细胞
Hepa1-6 小鼠肝癌细胞
HCT-8 人回盲肠癌细胞
BEL7402 人肝癌星空体育游戏下载官方
HaCaT 人永生化表皮细胞
GES-1 人胃上皮细胞
B16-F0 小鼠黑色素瘤细胞
AZ-521 人胃癌细胞
786-O 人肾透明细胞腺癌细胞
208F 大鼠成纤维细胞
293TN 人胚肾细胞--用于慢病毒包装
293FT 人胚肾细胞-用于慢病毒包装
GP-293 人胚肾细胞
293-L.P. 人胚肾细胞
HTB-55 Calu-3 人类肺腺癌 （胸水）
